Premium
Cell placement and guidance on substrates for neurochip interfaces
Author(s) -
Charrier Anne,
Martinez Dolores,
Monette Robert,
Comas Tanya,
Movileanu Raluca,
Py Christophe,
Denhoff Mike,
Krantis Anthony,
Mealing Geoff
Publication year - 2009
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.22539
Subject(s) - planar , patch clamp , interface (matter) , electrophysiology , nanotechnology , cell adhesion , materials science , chip , computer science , neuroscience , biophysics , cell , chemistry , biology , biochemistry , pulmonary surfactant , telecommunications , gibbs isotherm , computer graphics (images)
Interface devices such as integrated planar patch‐clamp chips are being developed to study the electrophysiological activity of neuronal networks grown in vitro. The utility of such devices will be dependent upon the ability to align neurons with interface features on the chip by controlling neuronal placement and by guiding cell connectivity. In this paper, we present a strategy to accomplish this goal. Patterned chemical modification of SiN surfaces with poly‐d‐lysine transferred from PDMS stamps was used to promote adhesion and guidance of cryo‐preserved primary rat cortical neurons. We demonstrate that these neurons can be positioned and grown over microhole features which will ultimately serve as patch‐clamp interfaces on the chip. Biotechnol. Bioeng. 2010; 105: 368–373. © 2009 Wiley Periodicals, Inc.