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Membrane technology for purification of therapeutic proteins
Author(s) -
Zydney Andrew L.
Publication year - 2009
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.22308
Subject(s) - ultrafiltration (renal) , downstream processing , membrane , recombinant dna , membrane fouling , membrane technology , chemistry , fouling , biology , biochemical engineering , chromatography , biochemistry , engineering , gene
Membrane systems are used throughout the downstream purification in the commercial production of high value therapeutic proteins. Over the last two decades, new membranes, modules, and systems have been developed specifically to meet the requirements of the biotechnology industry. These developments have been facilitated by an improved fundamental understanding of: (1) the effects of electrostatic interactions and concentration polarization on protein transmission during ultrafiltration and (2) the role of membrane morphology on protein fouling during both sterile and virus filtration. This perspective highlights some of the key work in this area and provides insights into possible future improvements in membrane technology for the purification of recombinant therapeutic proteins. Biotechnol. Bioeng. 2009;103: 227–230. © 2009 Wiley Periodicals, Inc.