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Metabolic flux analysis of Shewanella spp. reveals evolutionary robustness in central carbon metabolism
Author(s) -
Tang Yinjie J.,
Martin Hector Garcia,
Dehal Paramvir S.,
Deutschbauer Adam,
Llora Xavier,
Meadows Adam,
Arkin Adam,
Keasling Jay. D.
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.22129
Subject(s) - shewanella , facultative , biology , exponential growth , metabolic flux analysis , robustness (evolution) , metabolism , bacteria , metabolic pathway , flux (metallurgy) , chemostat , flux balance analysis , phylogenetic tree , biochemistry , ecology , chemistry , genetics , gene , mathematical analysis , mathematics , organic chemistry
Abstract Shewanella spp. are a group of facultative anaerobic bacteria widely distributed in marine and freshwater environments. In this study, we profiled the central metabolic fluxes of eight recently sequenced Shewanella species grown under the same condition in minimal medium with [3‐ 13 C] lactate. Although the tested Shewanella species had slightly different growth rates (0.23–0.29 h −1 ) and produced different amounts of acetate and pyruvate during early exponential growth (pseudo‐steady state), the relative intracellular metabolic flux distributions were remarkably similar. This result indicates that Shewanella species share similar regulation in regard to central carbon metabolic fluxes under steady growth conditions: the maintenance of metabolic robustness is not only evident in a single species under genetic perturbations (Fischer and Sauer, 2005; Nat Genet 37(6):636–640), but also observed through evolutionary related microbial species. This remarkable conservation of relative flux profiles through phylogenetic differences prompts us to introduce the concept of metabotype as an alternative scheme to classify microbial fluxomics. On the other hand, Shewanella spp. display flexibility in the relative flux profiles when switching their metabolism from consuming lactate to consuming pyruvate and acetate. Biotechnol. Bioeng. 2009;102: 1161–1169. © 2008 Wiley Periodicals, Inc.