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Selective separation and co‐culture of cells by photo‐induced enhancement of cell adhesion (PIECA)
Author(s) -
Edahiro Junichi,
Sumaru Kimio,
Ooshima Yuki,
Kanamori Toshiyuki
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.22124
Subject(s) - substrate (aquarium) , adhesion , cell culture , cell adhesion , biophysics , chemistry , biology , ecology , genetics , organic chemistry
Taking advantage of the phenomenon that animal cells adhering to a culture substrate are temporarily immobilized by light irradiation, we established a technique to manipulate the cells adhering to a culture substrate under microscopic observation. Using this technique, we demonstrated a separation of cells adhering to a culture substrate and fabrication of an elaborately patterned co‐culture system. Biotechnol. Bioeng. 2009;102: 1278–1282. © 2008 Wiley Periodicals, Inc.