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Photo‐immobilization of biological components on gold‐coated chips for measurements using surface plasmon resonance (SPR) and a quartz crystal microbalance (QCM)
Author(s) -
Tsuzuki Saki,
Wada Akira,
Ito Yoshihiro
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.22102
Subject(s) - quartz crystal microbalance , surface plasmon resonance , biomolecule , biosensor , chemistry , polyethylene glycol , polymer , bovine serum albumin , molecule , materials science , nanotechnology , chemical engineering , chromatography , nanoparticle , adsorption , organic chemistry , engineering
The photo‐immobilization technique is useful for immobilization of various biomolecules on assorted material surfaces, independent of the organic functional groups that may be present. Here, we report a convenient new photo‐immobilization technique that was developed by combining a nonbiofouling polymer containing polyethylene glycol and a photoreactive crosslinker for surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) measurements. By this method, nonspecific interactions were reduced and various types of molecules, bovine serum albumin, heparin, dsDNA, phosphatidylserine, Tobacco Mosaic Virus, and norfloxacine, were immobilized on an alkane thiol‐modified gold surface by a single method. The interactions of photo‐immobilized biomolecules and their corresponding antibodies were investigated by SPR and QCM. In addition, SPR imaging was possible using the present method. Biotechnol. Bioeng. 2009; 102: 700–707. © 2008 Wiley Periodicals, Inc.