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Recombinant mussel adhesive protein as a gene delivery material
Author(s) -
Hwang Dong Soo,
Kim Kyoung Ro,
Lim Seonghye,
Choi Yoo Seong,
Cha Hyung Joon
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.22086
Subject(s) - mussel , recombinant dna , adhesive , chemistry , gene delivery , gene , biology , biochemistry , genetic enhancement , fishery , organic chemistry , layer (electronics)
Efficient target gene delivery into eukaryotic cells is important for biotechnological research and gene therapy. Gene delivery based on proteins, including histones, has recently emerged as a powerful non‐viral DNA transfer technique. Here, we investigated the potential use of a recombinant mussel adhesive protein, hybrid fp‐151, as a gene delivery material, in view of its similar basic amino acid composition to histone proteins, and cost‐effective and high‐level production in Escherichia coli . After confirming DNA binding affinity, we transfected mammalian cells (human 293T and mouse NIH/3T3) with foreign genes using hybrid fp‐151 as the gene delivery carrier. Hybrid fp‐151 displayed comparable transfection efficiency in both mammalian cell lines, compared to the widely used transfection agent, Lipofectamine™ 2000. Our results indicate that this mussel adhesive protein may be used as a potential protein‐based gene‐transfer mediator. Biotechnol. Bioeng. 2009;102: 616–623. © 2008 Wiley Periodicals, Inc.