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Preparation of human immune effector T cells containing iron‐oxide nanoparticles
Author(s) -
Iida Hironori,
Takayanagi Kosuke,
Nakanishi Takuya,
Kume Akiko,
Muramatsu Kouji,
Kiyohara Yoshio,
Akiyama Yasuto,
Osaka Tetsuya
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21992
Subject(s) - magnetite , iron oxide nanoparticles , nanoparticle , endocytosis , immune system , iron oxide , ctl* , cytotoxic t cell , ferric , magnetic nanoparticles , chemistry , biophysics , materials science , nanotechnology , in vitro , biology , biochemistry , immunology , cell , inorganic chemistry , organic chemistry , metallurgy
Preparation of human immune T cells containing iron‐oxide nanoparticles was carried out for the development of magnetically mediated immunotherapy. Peripheral blood lymphocytes (PBLs) after the incubation with magnetite nanoparticles were found to contain measurable ferric ions, which suggested the incorporation of magnetite nanoparticles. Transmission electron microscopic (TEM) study indicated that the incorporation of magnetite nanoparticles was mediated by endocytosis of PBLs. Furthermore, the effects of dosages and diameter of magnetite nanoparticles on the magnetite incorporation were investigated, and it was demonstrated that the increase in dosage promoted the incorporation of nanoparticles and the uptake into PBLs was more effective for magnetite nanoparticles, which formed smaller aggregations in medium. Finally, the demonstration of magnetite incorporation into enriched T cells and tumor antigen‐specific cytotoxic T lymphocyte (CTL) line promises the achievement of magnetically mediated immunotherapy with tumor‐specific CTLs containing magnetic nanoparticles. Biotechnol. Bioeng. © 2008 Wiley Periodicals, Inc.