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Manufacture of stable palladium and gold nanoparticles on native and genetically engineered flagella scaffolds
Author(s) -
Deplanche Kevin,
Woods Richard D.,
Mikheenko Iryna P.,
Sockett R. Elizabeth,
Macaskie Lynne E.
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21966
Subject(s) - nanoparticle , protein filament , catalysis , flagellum , chemistry , sorption , nanotechnology , template , colloidal gold , palladium , chemical engineering , materials science , combinatorial chemistry , organic chemistry , biochemistry , adsorption , engineering , gene
The use of bacterial flagella as templates for the immobilization of Pd and Au nanoparticles is described. Complete coverage of D. desulfuricans flagellar filaments by Pd(0) nanoparticles was obtained via the H 2 ‐mediated reduction of [Pd(NH 3 ) 4 ]Cl 2 but similar results were not obtained using HAuCl 4 . The introduction of additional cysteine‐derived thiol residues in the E. coli FliC protein increased Au(III) sorption and reduction onto the surface of the flagellar filament and resulted in the production of stabilized Au(0) nanoparticles of ∼20–50 nm diameter. We demonstrate the application of molecular engineering techniques to manufacture biologically passivated Au(0) nanoparticles of a size suitable for catalytic applications. Biotechnol. Bioeng. © 2008 Wiley Periodicals, Inc.