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Determination of diauxic lag in continuous culture
Author(s) -
Lee DongUk,
Woo SungHoon,
Svoronos Spyros,
Koopman Ben
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21925
Subject(s) - lag , continuous flow , lag time , time lag , bacteria , biological system , growth rate , bacterial growth , replicate , bioreactor , chemistry , biology , biochemical engineering , mathematics , statistics , computer science , genetics , botany , computer network , engineering , geometry
A procedure was developed to characterize diauxic lag of bacteria switching between electron acceptors in continuous culture. In this procedure, a virtual batch growth curve is developed by integrating the time‐dependent net specific growth rates of bacteria observed under continuous flow conditions. The length of diauxic lag and the highest net specific growth rate following lag are conveniently estimated from the virtual batch curve. The procedure was found to give reproducible diauxic lag lengths and highest net specific growth rates when applied to experimental data from replicate continuous culture trials. Biotechnol. Bioeng. 2008;101: 642–646. © 2008 Wiley Periodicals, Inc.