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Role of methoxypolyethylene glycol on the hydration, activity, conformation and dynamic properties of a lipase in a dry film
Author(s) -
Secundo Francesco,
Barletta Gabriel,
Mazzola Giorgio
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21888
Subject(s) - chemistry , lipase , fluorescence spectroscopy , water activity , enzyme , random coil , organic chemistry , circular dichroism , biochemistry , fluorescence , water content , physics , geotechnical engineering , quantum mechanics , engineering
A combined approach based on the use of ATR‐FT/IR and steady‐state fluorescence spectroscopy allowed to shed light on the effects of the additive methoxypolyethylene glycol (MePEG) on the hydration, conformation and dynamic properties of lipase from Burkholderia cepacia dehydrated to form a film. Spectroscopic data show that the additive has little effect on the structure of the protein; however, H/D exchange kinetic and fluorescence anisotropy suggest a more flexible enzyme molecule when in the presence of MePEG. By infrared spectroscopy, we estimated that, after conditioning the films at water activity of 1, the water content in the lipase dehydrated with MePEG is 5.4‐ and 4.7‐fold higher than in the absence of the additive and the additive alone, respectively. Additionally, our infrared data suggest that MePEG acts by hindering intermolecular protein–protein interactions and contributing to increase the accessibility and flexibility of the lipase in the dehydrated solid film. These factors also explain the enhancement of the enzyme catalytic activity (i.e., up to 3.7‐fold in neat organic solvent) when in the presence of MePEG. The method and results presented might better address the use of additives for the preparation of enzymes employed in non‐aqueous media or of proteins used in a dry form in different fields of biotechnology. Biotechnol. Bioeng. 2008;101: 255–262. © 2008 Wiley Periodicals, Inc.