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Tuning the expression level of recombinant proteins by modulating mRNA stability in a cell‐free protein synthesis system
Author(s) -
Ahn JinHo,
Kang TaekJin,
Kim DongMyung
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21884
Subject(s) - terminator (solar) , messenger rna , protein biosynthesis , recombinant dna , microbiology and biotechnology , chemistry , cell free protein synthesis , biology , biochemistry , gene , physics , ionosphere , astronomy
In this work, it was discovered that the stability of mRNA in a cell‐free extract could be controlled by using engineered T7 terminator sequences. Specifically, it was found that mRNA stability gradually decreased as the length of the stem structure of the T7 terminator was reduced sequentially. As a result of the controlled abundance of mRNA species, it was possible to manipulate the relative expression level of target proteins by employing the T7 terminator of adjusted stem lengths. Biotechnol. Bioeng. 2008;101: 422–427. © 2008 Wiley Periodicals, Inc.