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Chromatographic separation of three monoclonal antibody variants using multicolumn countercurrent solvent gradient purification (MCSGP)
Author(s) -
MüllerSpäth Thomas,
Aumann Lars,
Melter Lena,
Ströhlein Guido,
Morbidelli Massimo
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21843
Subject(s) - countercurrent exchange , chromatography , chemistry , yield (engineering) , solvent , separation process , simulated moving bed , fraction (chemistry) , process (computing) , monoclonal antibody , countercurrent chromatography , high performance liquid chromatography , materials science , thermodynamics , organic chemistry , antibody , adsorption , biology , computer science , immunology , metallurgy , operating system , physics
Multicolumn countercurrent solvent gradient purification (MCSGP) is a continuous chromatographic process developed in recent years (Aumann and Morbidelli, 2007a; Aumann et al., 2007) that is particularly suited for applications in the field of bioseparations. Like batch chromatography, MCSGP is suitable for three‐fraction chromatographic separations and able to perform solvent gradients but it is superior in terms of solvent consumption, yield, purity, and productivity due to the countercurrent movement of the liquid and the solid phases. In this work, the MCSGP process is applied to the separation of three monoclonal antibody variants on a conventional preparative cation exchange resin. The experimental process performance was compared to simulations based on a lumped kinetic model. Yield and purity values of the target variant of 93%, respectively were obtained experimentally. The batch reference process was clearly outperformed by the MCSGP process. Biotechnol. Bioeng. 2008;100: 1166–1177. © 2008 Wiley Periodicals, Inc.