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Pressure‐driven perfusion culture microchamber array for a parallel drug cytotoxicity assay
Author(s) -
Sugiura Shinji,
Edahiro Junichi,
Kikuchi Kyoko,
Sumaru Kimio,
Kanamori Toshiyuki
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21836
Subject(s) - cytotoxicity , perfusion , drug , chemistry , biomedical engineering , cardiology , pharmacology , medicine , in vitro , biochemistry
Abstract This article reports a pressure‐driven perfusion culture chip developed for parallel drug cytotoxicity assay. The device is composed of an 8 × 5 array of cell culture microchambers with independent perfusion microchannels. It is equipped with a simple interface for convenient access by a micropipette and connection to an external pressure source, which enables easy operation without special training. The unique microchamber structure was carefully designed with consideration of hydrodynamic parameters and was fabricated out of a polydimethylsiloxane by using multilayer photolithography and replica molding. The microchamber structure enables uniform cell loading and perfusion culture without cross‐contamination between neighboring microchambers. A parallel cytotoxicity assay was successfully carried out in the 8 × 5 microchamber array to analyze the cytotoxic effects of seven anticancer drugs. The pressure‐driven perfusion culture chip, with its simple interface and well‐designed microfluidic network, will likely become an advantageous platform for future high‐throughput drug screening by microchip. Biotechnol. Bioeng. 2008;100: 1156–1165. © 2008 Wiley Periodicals, Inc.