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Multiplex bacterial growth monitoring in 24‐well microplates using a dual optical sensor for dissolved oxygen and pH
Author(s) -
Kocincová Anna S.,
Nagl Stefan,
Arain Sarina,
Krause Christian,
Borisov Sergey M.,
Arnold Matthias,
Wolfbeis Otto S.
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21793
Subject(s) - multiplex , oxygen , dual (grammatical number) , oxygen sensor , chemistry , bacterial growth , environmental chemistry , chromatography , analytical chemistry (journal) , chemical engineering , bacteria , biology , organic chemistry , bioinformatics , genetics , engineering , art , literature
Abstract Non‐invasive, simultaneous optical monitoring of oxygen and pH during bacterial cultivation in 24‐well microplates is presented using an integrated dual sensor for dissolved oxygen and pH values. The dual sensor is based on oxygen‐sensitive organosilica microparticles and pH‐sensitive microbeads from a polymethacrylate derivative embedded into a polyurethane hydrogel. The readout is based on a phase‐domain fluorescence lifetime‐based method referred to as modified frequency domain dual lifetime referencing using a commercially available detector system for 24‐well microplates. The sensor was used for monitoring the growth of Pseudomonas putida bacterial cultures. The method is suitable for parallelized, miniaturized bioprocessing, and cell‐based high‐throughput screening applications. Biotechnol. Bioeng. 2008;100: 430–438. © 2008 Wiley Periodicals, Inc.