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Live cells‐based cytotoxic sensorchip fabricated in a microfluidic system
Author(s) -
Wada KenIchi,
Taniguchi Akiyoshi,
Kobayashi Jun,
Yamato Masayuki,
Okano Teruo
Publication year - 2007
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21718
Subject(s) - cytotoxic t cell , microfluidics , green fluorescent protein , transfection , reagent , chemistry , laminar flow , nanotechnology , fluorescence , biophysics , microbiology and biotechnology , materials science , biology , in vitro , biochemistry , gene , physics , quantum mechanics , thermodynamics
We report here a live cells‐based sensorchip fabricated in microfluidic channels in which several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line, which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat shock protein 70B′ (HSP70B′) promoter, which is induced by cytotoxic reagents. The fluorescence in the sensor cells increased in a CdCl 2 dose‐dependent manner in the microfluidic channels. In this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive and high‐throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of micro‐total analysis systems (µ‐TAS) technology, and can be applied to toxicology, environmental assessment and drug screening. Biotechnol. Bioeng. 2008;99: 1513–1517. © 2007 Wiley Periodicals, Inc.