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Growth and cometabolic reduction kinetics of a uranium‐ and sulfate‐reducing Desulfovibrio /Clostridia mixed culture: Temperature effects
Author(s) -
Boonchayaanant Benjaporn,
Kitanidis Peter K.,
Criddle Craig S.
Publication year - 2007
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21670
Subject(s) - kinetics , clostridia , chemistry , uranium , bioremediation , environmental chemistry , desulfovibrio , sulfate , biology , contamination , bacteria , ecology , organic chemistry , materials science , physics , quantum mechanics , metallurgy , genetics
Bioremediation of contaminated soils and aquifers is subject to spatial and temporal temperature changes that can alter the kinetics of key microbial processes. This study quantifies temperature effects on the kinetics of an ethanol‐fed sulfate‐reducing mixed culture derived from a uranium‐contaminated aquifer subject to seasonal temperature fluctuations. The mixed culture contains Desulfovibrio sp. and a Clostridia‐like organism. Rates of growth, ethanol utilization, decay, and uranium reduction decreased with decreasing temperature. No significant uranium reduction was observed at 10°C. While both Monod saturation kinetics and pseudo second‐order kinetics adequately described the rates of growth and utilization of electron donor (ethanol), model parameters for the pseudo second‐order expression had smaller uncertainties. Uranium reduction kinetics were best described by pseudo second‐order kinetics modified to include a term for inactivation/death of cells. Biotechnol. Bioeng. 2008;99: 1107–1119. © 2007 Wiley Periodicals, Inc.