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Lab‐scale fermentation tests of microchip with integrated electrochemical sensors for pH, temperature, dissolved oxygen and viable biomass concentration
Author(s) -
Krommenhoek Erik E.,
van Leeuwen Michiel,
Gardeniers Han,
van Gulik Walter M.,
van den Berg Albert,
Li Xiaonan,
Ottens Marcel,
van der Wielen Luuk A.M.,
Heijnen Joseph J.
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21661
Subject(s) - microelectrode , oxygen sensor , biomass (ecology) , detection limit , analytical chemistry (journal) , chemistry , dielectric spectroscopy , oxygen , electrochemistry , materials science , electrode , chromatography , organic chemistry , oceanography , geology
Abstract This article shows the development and testing of a microchip with integrated electrochemical sensors for measurement of pH, temperature, dissolved oxygen and viable biomass concentration under yeast cultivation conditions. Measurements were done both under dynamic batch conditions as well as under prolonged continuous cultivation conditions. The response of the sensors compared well with conventional measurement techniques. The biomass sensor was based on impedance spectroscopy. The results of the biomass sensor matched very well with dry weight measurements and showed a limit of detection of ∼1 g/L. The dissolved oxygen concentration was monitored amperometrically using an ultra‐microelectrode array, which showed an accuracy of ∼0.2 mg/L and negligible drift. pH was monitored using an ISFET with an accuracy well below 0.1 pH unit. The platinum thin‐film temperature resistor followed temperature changes with ∼0.1°C accuracy. The dimensions of the multi sensor chip are chosen as such that it is compatible with the 96‐well plate format. Biotechnol. Bioeng. 2008;99: 884–892. © 2007 Wiley Periodicals, Inc.