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Comparison of two‐phase lipase‐catalyzed esterification on micro and bench scale
Author(s) -
Swarts Jan W.,
Vossenberg Petra,
Meerman Marieke H.,
Janssen Anja E.M.,
Boom Remko M.
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21650
Subject(s) - lipase , candida antarctica , catalysis , chemistry , reagent , enzyme , butanol , alcohol , phase (matter) , decane , scale (ratio) , organic chemistry , chemical engineering , chromatography , ethanol , physics , quantum mechanics , engineering
Lipase type B from Candida antarctica was used to catalyze the esterification of propionic acid and 1‐butanol in a water/ n ‐decane two‐phase system on micro and on bench scale. The reaction was described by a Ping Pong Bi Bi mechanism with alcohol inhibition. The kinetic parameters on micro and bench scale were compared; no significant differences were found. Furthermore, effects of temperature on activation and inactivation of the enzyme were found to be similar on micro and bench scale. Therefore, parameters found on either scale can be used for the other scale. Enzyme kinetic parameters can be determined on a micro scale, with very low consumption of reagents and catalyst, and then be applied to bench scale. This can reduce the cost of optimizing enzyme processes by downscaling. Biotechnol. Bioeng. 2008;99: 855–861. © 2007 Wiley Periodicals, Inc.