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Engineering poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate) copolymer composition in E. coli
Author(s) -
Wong Matthew S.,
Causey Thomas B.,
Mantzaris Nikos,
Bennett George N.,
San KaYiu
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21641
Subject(s) - polyhydroxyalkanoates , copolymer , operon , escherichia coli , composition (language) , strain (injury) , chemistry , raw material , biosynthesis , plasmid , dispersity , metabolic engineering , biochemistry , polyhydroxybutyrate , bacteria , food science , polymer , biology , enzyme , polymer chemistry , organic chemistry , gene , genetics , linguistics , philosophy , anatomy
Abstract A strain of Escherichia coli was metabolically engineered to produce poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate) (PHBV) of specified composition between 5% and 18% HV. A gene encoding propionyl‐CoA synthetase ( prpE from S. enterica ) was placed under the control of the IPTG‐inducible tac promoter (P taclacUV5 ) while the polyhydroxyalkanoate synthesis operon ( phaBCA ) from R. eutropha was expressed constitutively. A strain of E. coli harboring both plasmids was grown in defined medium and PHBV was produced with specified hydroxyvalerate (HV) molar content between 5% and 18%. The molecular weight of the copolymer was ∼700,000 across various HV contents, and average polydispersity was ∼ 1.3. The majority of the PHBV production occurred during the late exponential/stationary phase. The HV content of the copolymer generally peaked early in the incubation before falling to its final value. We found that the time profiles of PrpE activity, propionyl‐CoA, and acetyl‐CoA were well correlated to the HV content time profile. Despite an abundance of propionyl‐CoA, incorporation of HV into the copolymer was inefficient. Therefore, both the PHA operon and conditions affecting the availability of propionyl‐CoA must be chosen carefully to achieve the desired HV content. The ability to engineer copolymer composition control into an E. coli strain would be useful in cases where the feedstock composition is not adjustable. Biotechnol. Bioeng. 2008;99: 919–928. © 2007 Wiley Periodicals, Inc.