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Prediction of diffusion coefficients of plasmids
Author(s) -
Prazeres D.M.F.
Publication year - 2008
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21626
Subject(s) - plasmid , diffusion , chemistry , correlation coefficient , molecule , base (topology) , biological system , chromatography , dna , analytical chemistry (journal) , materials science , thermodynamics , biology , physics , mathematics , statistics , biochemistry , organic chemistry , mathematical analysis
The ability to predict diffusion coefficients is important in the design, analysis, and operation of plasmid downstream processing operations such as membrane and fixed‐bed chromatography. A correlation is proposed to predict the diffusion coefficient, D , of supercoiled plasmid DNA molecules in dilute solutions on the basis of the molecular weight, M , or size. Experimental data (18 points) collected from the literature confirmed the proposed variation of D with plasmid molecular weight as D ∝ M −2/3 , for molecules within the 1,800–287,100 base‐pair range. The correlation was able to estimate the available experimental results with an average error of 6.3%. Biotechnol. Bioeng. 2008; 99; 1040–1044. © 2007 Wiley Periodicals, Inc.