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Accelerated cell line development using two‐color fluorescence activated cell sorting to select highly expressing antibody‐producing clones
Author(s) -
Sleiman Robert J.,
Gray Peter P.,
McCall Martin N.,
Codamo Joe,
Sunstrom NoelleAnn S.
Publication year - 2007
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21612
Subject(s) - chinese hamster ovary cell , cell sorting , antibody , cell culture , transfection , monoclonal antibody , recombinant dna , biology , green fluorescent protein , microbiology and biotechnology , computational biology , flow cytometry , biochemistry , gene , immunology , genetics
The success of engineered monoclonal antibodies as biopharmaceuticals has generated considerable interest in strategies designed to accelerate development of antibody expressing cell lines. Stable mammalian cell lines that express therapeutic antibodies at high levels typically take 6–12 months to develop. Here we describe a novel method to accelerate selection of cells expressing recombinant proteins (e.g., antibodies) using multiparameter fluorescence activated cell sorting (FACS) in association with dual intracellular autofluorescent reporter proteins. The method is co‐factor‐independent and does not require complex sample preparation. Chinese hamster ovary (CHO) clones expressing high levels of recombinant antibody were selected on the basis of a two‐color FACS sorting strategy using heavy and light chain‐specific fluorescent reporter proteins. We were able to establish within 12 weeks of transfection cell lines with greater than a 38‐fold increase in antibody production when compared to the pool from which they were isolated, following a single round of FACS. The method provides a robust strategy to accelerate selection and characterization of clones and builds a foundation for a predictive model of specific productivity based upon on two‐color fluorescence. Biotechnol. Bioeng. 2008;99: 578–587. © 2007 Wiley Periodicals, Inc.