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A semicontinuous 3‐column countercurrent solvent gradient purification (MCSGP) process
Author(s) -
Aumann Lars,
Morbidelli Massimo
Publication year - 2007
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21585
Subject(s) - countercurrent exchange , chromatography , process (computing) , column (typography) , chemistry , solvent , unit operation , countercurrent chromatography , process engineering , column chromatography , computer science , chemical engineering , thermodynamics , organic chemistry , engineering , physics , telecommunications , frame (networking) , operating system
The recently developed continuous Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) Process has been reduced to a fully equivalent semicontinuous setup with only three chromatographic columns and three gradient pump modules. Actually the 3‐column MCSGP unit can even achieve better performance than the original 6‐column process due to an additional degree of freedom, that is a different switching time for the “batch lane” and the “interconnected lane.” Experimental results for the 3‐column MCSGP unit of the purification of an industrial multicomponent peptide mixture containing 46% of Calcitonin on a reversed phase resin are compared with model simulations. It is concluded, that the model is well suited to predict the system behavior and therefore to design its optimal operating conditions. Biotechnol. Bioeng. 2008;99: 728–733. © 2007 Wiley Periodicals, Inc.