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Adhesion and viability of waterborne pathogens on p‐DADMAC coatings
Author(s) -
van der Mei Henny C.,
RustemaAbbing Minie,
Langworthy Don E.,
Collias Dimitris I.,
Mitchell Michael D.,
Bjorkquist Dave W.,
Busscher Henk J.
Publication year - 2007
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21538
Subject(s) - adhesion , chemistry , coating , aqueous solution , escherichia coli , microbiology and biotechnology , surface charge , ammonium chloride , biochemistry , organic chemistry , biology , gene
The attachment of waterborne pathogens onto surfaces can be increased by coating the surfaces with positive charge‐enhancing polymers. In this paper, the increased efficacy of polydiallyldimethylammonium chloride (p‐DADMAC) coatings on glass was evaluated in a parallel plate flow chamber with the use of waterborne pathogens ( Raoultella terrigena , Escherichia coli , and Brevundimonas diminuta ). p‐DADMAC coatings strongly compensated the highly negative charges on the glass surface and even yielded a positively charged surface when applied from a 500 ppm solution. Whereas none of the strains adhered from water to glass due to electrostatic repulsion, R. terrigena and E. coli readily adhered in high numbers to p‐DADMAC coated glass slides applied from 1, 100, or 500 ppm aqueous solutions. B. diminuta only adhered to a positively charged p‐DADMAC coating applied from a 500 ppm solution. In addition, all p‐DADMAC coatings indicated strong contact killing with the bacterial species used in this study by live/dead staining techniques. In summary, this paper demonstrates the potential of p‐DADMAC coatings to strongly enhance bacterial adhesion. Moreover, once adhered, bacterial viability can be reduced by the positively charged ammonium groups in the coating. Biotechnol. Bioeng. 2008;99: 165–169. © 2007 Wiley Periodicals, Inc.