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Optimal thermotolerance of Bifidobacterium bifidum in gellan–alginate microparticles
Author(s) -
Chen MingJu,
Chen KunNan,
Kuo YiTzu
Publication year - 2007
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21450
Subject(s) - bifidobacterium bifidum , gellan gum , bifidobacterium , actinomycetaceae , microbiology and biotechnology , chemistry , biology , food science , lactobacillus , fermentation
The purpose of this research was to encapsulate Bifidobacterium bifidum using gellan, sodium alginate and prebiotics as coating materials, and to maximize the thermotolerance of the probiotics with an optimal combination of the coating materials. The optimal ratio of the coating materials for the microparticles under heat treatments (75°C, 1 min) was obtained by using the response surface method and the sequential quadratic programming technique. Optimization results indicated that 2% sodium alginate mixed with 1% gellan gum as coating materials would produce the highest thermotolerance in terms of B. bifidum count. The verification experiment yielded a result close to the predicted values, with no significant difference ( P > 0.05). The results of heat treatments also demonstrated that the addition of gellan gum in the walls of probiotic microcapsules provided improved protection for B. bifidum . These probiotic counts remained at 10 5 –10 6 CFU/g for the microcapsules stored for 2 months, then treated in heat and in simulated gastric fluid. Biotechnol. Bioeng. 2007;98: 411–419. © 2007 Wiley Periodicals, Inc.