Silica‐immobilized enzymes for multi‐step synthesis in microfluidic devices | Zendy

Luckarift Heather R. | Zendy; Ku Bosung S. | Zendy; Dordick Jonathan S. | Zendy; Spain Jim C. | Zendy

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Silica‐immobilized enzymes for multi‐step synthesis in microfluidic devices

Author(s) -

Luckarift Heather R.,

Ku Bosung S.,

Dordick Jonathan S.,

Spain Jim C.

Publication year - 2007

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.21447

Subject(s) - microfluidics , immobilized enzyme , nitrobenzene , chemistry , biocatalysis , microreactor , zinc , polymerization , substrate (aquarium) , combinatorial chemistry , enzyme , chromatography , nanotechnology , organic chemistry , catalysis , materials science , reaction mechanism , polymer , oceanography , geology

The combinatorial synthesis of 2‐aminophenoxazin‐3‐one (APO) in a microfluidic device is reported. Individual microfluidic chips containing metallic zinc, silica‐immobilized hydroxylaminobenzene mutase and silica‐immobilized soybean peroxidase are connected in series to create a chemo‐enzymatic system for synthesis. Zinc catalyzes the initial reduction of nitrobenzene to hydroxylaminobenzene which undergoes a biocatalytic conversion to 2‐aminophenol, followed by enzymatic polymerization to APO. Silica‐immobilization of enzymes allows the rapid stabilization and integration of the biocatalyst within a microfluidic device with minimal preparation. The system proved suitable for synthesis of a complex natural product (APO) from a simple substrate (nitrobenzene) under continuous flow conditions. Biotechnol. Bioeng. 2007;98: 701–705. © 2007 Wiley Periodicals, Inc.

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