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Process development for the electroenzymatic synthesis of ( R )‐methylphenylsulfoxide by use of a 3‐dimensional electrode
Author(s) -
Lütz S.,
Vuorilehto K.,
Liese A.
Publication year - 2007
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21434
Subject(s) - thioanisole , hydrogen peroxide , yield (engineering) , enantioselective synthesis , electrochemistry , cathode , biotransformation , chemistry , electrosynthesis , graphite , electrode , catalysis , combinatorial chemistry , organic chemistry , materials science , enzyme , metallurgy
Abstract The electrogeneration of hydrogen peroxide via reduction of dissolved oxygen was carried out in a three‐dimensional electrochemical cell using graphite grains as cathode material and combined with the enantioselective oxidation of thioanisole to ( R )‐methylphenylsulfoxide catalyzed by Chloroperoxidase from Caldariomyces fumago . The relevant parameters for process development (e.g., conductivity of the biotransformation medium) were identified and optimized, leading to an efficient electroenzymatic process with a space‐time yield of 104 g · L −1 · d −1 . The isolated product was produced on gram scale (1.2 g, purity >98%, ee > 98.5%). Biotechnol. Bioeng. 2007;98: 525–534. © 2007 Wiley Periodicals, Inc.