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Correlating single cell motility with population growth dynamics for flagellated bacteria
Author(s) -
Arora Sucheta,
Bhat Vidya,
Mittal Aditya
Publication year - 2007
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21372
Subject(s) - motility , bioreactor , bacteria , population , biology , chemotaxis , biofilm , bacterial growth , bioprocess , video microscopy , microbiology and biotechnology , escherichia coli , biological system , chemistry , biophysics , biochemistry , genetics , paleontology , botany , demography , receptor , sociology , gene
Many bacteria used for biotechnological applications are naturally motile. Their “bio‐nanopropeller” driven movement allows searching for better environments in a process called chemotaxis. Since bacteria are extremely small in size compared to the bulk fluid volumes in bioreactors, single cell motility is not considered to influence bioreactor operations. However, with increasing interest in localized fluid flow inside reactors, it is important to ask whether individual motility characteristics of bacteria are important in bioreactor operations. The first step in this direction is to try to correlate single cell measurements with population data of motile bacteria in a bioreactor. Thus, we observed the motility behavior of individual bacterial cells, using video microscopy with 33 ms time resolution, as a function of population growth dynamics of batch cultures in shake flasks. While observing the motility behavior of the most intensively studied bacteria, Escherichia coli , we find that overall bacterial motility decreases with progression of the growth curve. Remarkably, this is due to a decrease in a specific motility behavior called “running”. Our results not only have direct implications on biofilm formations, but also provide a new direction in bioprocess design research highlighting the role of individual bacterial cell motility as an important parameter. Biotechnol. Bioeng. 2007; 97: 1644–1649. © 2007 Wiley Periodicals, Inc.