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Combined approach of NMR and chemometrics for screening peptones used in the cell culture medium for the production of a recombinant therapeutic protein
Author(s) -
Luo Ying,
Chen Guoxiang
Publication year - 2007
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21365
Subject(s) - chemometrics , biochemical engineering , chemistry , recombinant dna , nuclear magnetic resonance spectroscopy , hydrolysate , microbiology and biotechnology , food science , chromatography , process engineering , biochemistry , biology , organic chemistry , engineering , hydrolysis , gene
Soy peptones or soy hydrolysates are widely used as key medium additives in serum‐free cell culture processes for industrial production of therapeutic recombinant proteins. The heterogeneous nature of these vegetable‐derived materials can lead to substantial lot‐ to‐lot variability in cell culture processes. In this study, we demonstrated the feasibility of nuclear magnetic resonance (NMR) spectroscopy in combination with chemometrics in rapid screening peptone lots in order to optimize efficiency and consistency of large‐scale protein production. This report is the first that shows a correlation between the intrinsic NMR spectral characteristics of complex heterogeneous materials and product titer using chemometrics. Biotechnol. Bioeng. 2007; 97: 1654–1659. © 2007 Wiley Periodicals, Inc.