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An aerobic sequencing batch reactor for 2,4,6‐trinitrophenol (picric acid) biodegradation
Author(s) -
Weidhaas Jennifer L.,
Schroeder Edward D.,
Chang Daniel P.Y.
Publication year - 2007
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21361
Subject(s) - biodegradation , sequencing batch reactor , picric acid , chemistry , batch reactor , wastewater , bioreactor , degradation (telecommunications) , nuclear chemistry , chromatography , environmental chemistry , pulp and paper industry , organic chemistry , environmental engineering , environmental science , computer science , engineering , catalysis , telecommunications
A bench scale sequencing batch reactor (SBR) was designed and tested for degradation of 2,4,6‐trinitrophenol (TNP) or picric acid‐contaminated wastewater or groundwater. Under varying temperatures (25, 15 and 10°C) and influent concentrations (40–200 mg/ L TNP) a stable biomass was developed that was consistently capable of degrading the explosive compound to below regulatory drinking water limits (0.057 mg/ L ). The reactor was initially seeded with a nitroaromatic and nitramine degrading isolate Rhodococcus opacus strain JW01. Kinetic growth modeling was conducted revealing µ max values at 25, 15 and 10°C of 0.14, 0.08 and 0.04 d −1 , while the modeled K s values were 0.68, 1.11 and 1.24 mg/ L , respectively. Overall TNP removal efficiency in the SBR was on average > 99.9% over the 2000 hours of operation. Removal of TNP to below drinking water standards, with low residual dissolved carbon and significant release of nitrogen from the parent compound was accomplished. Biotechnol. Bioneg. 2007;97: 1408–1414. © 2007 Wiley Periodicals, Inc.