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Proteome analysis of heat shock protein expression in Pseudomonas alcaligenes NCIMB 9867 in response to gentisate exposure and elevated growth temperature
Author(s) -
Zhao Bing,
Yeo Chew Chieng,
Tan Chew Ling,
Poh Chit Laa
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21253
Subject(s) - alcaligenes , microbiology and biotechnology , heat shock , proteome , biology , heat shock protein , protein expression , pseudomonadaceae , pseudomonadales , pseudomonas , bacteria , biochemistry , genetics , gene
Pseudomonas alcaligenes NCIMB 9867 (strain P25X) degrades xylenols and cresols via the gentisate pathway. P25X expresses two isofunctional gentisate 1,2‐dioxygenases (GDO I and GDO II). The expression of both GDOs was not detected when P25X cells were grown at 42°C, even in the presence of gentisate. A total of 19 heat shock proteins (Hsps) belonging to the Hsp100, Hsp90, Hsp70, Hsp60, Hsp45, and small heat shock protein (sHsp) families were identified among the protein spots that were either newly detected or were expressed at levels of at least twofold higher when P25X cells were cultured at 32 or 42°C in the presence and absence of gentisate. Among these, 16 Hsps were commonly expressed at 42°C. Two additional Hsps (H5 and H13) from the Hsp90 and Hsp60 families, respectively, were expressed only when P25X cells were grown at 42°C and in the presence of gentisate. A protein of the sHsp (H16) family was expressed only in the presence of gentisate at 32°C but not at 42°C. The GroEL chaperonins of the Hsp60 family comprised the largest group of Hsps identified and exhibited high level of expression at 42°C following gentisate exposure. Biotechnol. Bioeng. 2007;97: 506–514. © 2006 Wiley Periodicals, Inc.