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Measurement of fast dynamic intracellular pH in Saccharomyces cerevisiae using benzoic acid pulse
Author(s) -
Kresnowati M.T.A.P.,
SuarezMendez C.,
Groothuizen M.K.,
van Winden W.A.,
Heijnen J.J.
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21179
Subject(s) - intracellular , benzoic acid , saccharomyces cerevisiae , intracellular ph , chemistry , yeast , pulse (music) , metabolism , biochemistry , kinetics , biophysics , chromatography , biology , computer science , quantum mechanics , detector , telecommunications , physics
Abstract pH affects many processes on cell metabolism, such as enzyme kinetics. To enhance the understanding of the living cells, it is therefore indispensable to have a method to monitor the pH in living cells. To accomplish this, a dynamic intracellular pH measurement method applying low concentration benzoic acid pulse was developed. The method was thoroughly validated and successfully implemented for measuring fast dynamic intracellular pH of Saccharomyces cerevisiae in response to a glucose pulse perturbation performed in the BioSCOPE set‐up. Fast drop in intracellular pH followed by partial alkalinization was observed following the pulse. The low concentration benzoic acid pulse which was implemented in the method avoids the undesirable effects that may be introduced by benzoic acid to cell metabolism. Biotechnol. Bioeng. 2007;97:86–98. © 2006 Wiley Periodicals, Inc.