Quantitative real‐time PCR for rapid and accurate titration of recombinant baculovirus particles | Zendy

Hitchman Richard B. | Zendy; Siaterli Evangelia A. | Zendy; Nixon Clare P. | Zendy; King Linda A. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Quantitative real‐time PCR for rapid and accurate titration of recombinant baculovirus particles

Author(s) -

Hitchman Richard B.,

Siaterli Evangelia A.,

Nixon Clare P.,

King Linda A.

Publication year - 2006

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.21177

Subject(s) - titration , recombinant dna , chromatography , real time polymerase chain reaction , chemistry , baculoviridae , virology , biology , biochemistry , gene , organic chemistry , spodoptera

We describe the use of quantitative PCR (QPCR) to titer recombinant baculoviruses. Custom primers and probe were designed to gp64 and used to calculate a standard curve of QPCR derived titers from dilutions of a previously titrated baculovirus stock. Each dilution was titrated by both plaque assay and QPCR, producing a consistent and reproducible inverse relationship between C T and plaque forming units per milliliter. No significant difference was observed between titers produced by QPCR and plaque assay for 12 recombinant viruses, confirming the validity of this technique as a rapid and accurate method of baculovirus titration. Biotechnol. Bioeng. 2007;96:810–814. © 2006 Wiley Periodicals, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research