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The IE2 promoter/enhancer region from mouse CMV provides high levels of therapeutic protein expression in mammalian cells
Author(s) -
Chatellard Philippe,
Pankiewicz Renata,
Meier Evelyne,
Durrer Laurence,
Sauvage Christophe,
Imhof Markus O.
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21172
Subject(s) - enhancer , biology , gene , human cytomegalovirus , protein expression , promoter , gene expression , computational biology , microbiology and biotechnology , genetics
Protein expression in mammalian cells is key for the production and manufacturing of bio‐therapeutics with human‐like properties and activities. As a molecular basis for reaching high protein expression levels, efficient promoter/enhancer systems are a prerequisite. Here we identify a novel enhancer from the mouse cytomegalovirus (CMV) immediate early 2 (IE2) region as a strong expression‐promoting element. We further demonstrate its activity in bi‐directional promoter architecture and apply it to generate production clones for IL‐18BP, a protein with therapeutic indications in autoimmune diseases. These data show that the IE region from mouse CMV, and the IE2 enhancer/promoter in particular, have a broad potential for application in novel gene expression systems for research, development, and manufacturing of protein drugs. Biotechnol. Bioeng. 2007;96: 106–117. © 2006 Wiley Periodicals, Inc.