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Using an aerosol deposition model to increase hairy root growth in a mist reactor
Author(s) -
Towler Melissa J.,
Wyslouzil Barbara E.,
Weathers Pamela J.
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21143
Subject(s) - mist , aerosol , biomass (ecology) , growth rate , chemistry , deposition (geology) , carbon fibers , nutrient , sucrose , flux (metallurgy) , bioreactor , food science , materials science , agronomy , biology , meteorology , organic chemistry , paleontology , physics , geometry , mathematics , sediment , composite number , composite material
Gas‐phase reactors, including the mist reactor, have distinct advantages over liquid‐phase reactors including the ability to manipulate the gas composition, to allow effective gas exchange in a densely growing biomass, and to affect secondary metabolite production. Mathematical modeling suggested that roots in a mist reactor are often too sparsely packed to capture mist particles efficiently and cannot, therefore, meet the nutrient demands required to maintain high growth rates. Indeed, growth rates of Artemisia annua hairy roots increased significantly when the initial packing density increased or when a higher sucrose concentration was used in the medium. Growth kinetics for 2, 4, and 6 days, however, showed a decrease or stationary growth rate after only 4 days for both 3 and 5% sucrose feeds. Residual medium analyses indicated that carbon was not exhausted, nor were any of the other major nutrients including phosphate. Increasing the mist duty cycle at constant carbon flux through the reactor reduced the growth rates slightly. In general, the aerosol deposition model correctly predicted how to optimize hairy root growth in the mist reactor. Biotechnol. Bioeng. 2007;96:881–891. © 2006 Wiley Periodicals, Inc.

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