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Influence of pH, temperature, and urea molar flowrate on Arthrospira platensis fed‐batch cultivation: A kinetic and thermodynamic approach
Author(s) -
SánchezLuna Luis Dante,
Bezerra Raquel Pedrosa,
Matsudo Marcelo Chuei,
Sato Sunao,
Converti Attilio,
de Carvalho João Carlos Monteiro
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21097
Subject(s) - response surface methodology , central composite design , nitrogen , chemistry , factorial experiment , urea , volumetric flow rate , analytical chemistry (journal) , chromatography , thermodynamics , biochemistry , organic chemistry , mathematics , statistics , physics
Arthrospira platensis was cultivated photoautotrophically at 6.0 klux light intensity in 5.0‐L open tanks, using a mineral medium containing urea as nitrogen source. Fed‐batch experiments were performed at constant flowrate. A central composite factorial design combined to response surface methodology (RSM) was utilized to determine the relationship between the selected response variables (cell concentration after 10 days, X m , cell productivity, P X , and nitrogen‐to‐cell conversion factor, Y X / N ) and codified values of the independent variables (pH, temperature, T , and urea flowrate, K ). By applying the quadratic regression analysis, the equations describing the behaviors of these responses as simultaneous functions of the selected independent variables were determined, and the conditions for X m and P X optimization were estimated (pH 9.5, T = 29°C, and K = 0.551 mM/day). The experimental data obtained under these conditions ( X m = 749 mg/L; P X = 69.9 mg/L·day) were very close to the estimated ones ( X m = 721 mg/L; P X = 67.1 mg/L·day). Additional cultivations were carried out under the above best conditions of pH control and urea flowrate at variable temperature. Consistently with the results of RSM, the best growth temperature was 29°C. The maximum specific growth rates at different temperatures were used to estimate the thermodynamic parameters of growth (Δ H * = 59.3 kJ/mol; Δ S* = −0.147 kJ/mol·K; Δ G* = 103 kJ/mol) and its thermal inactivation (Δ H D o = 72.0 kJ/mol; Δ S D o = 0.144 kJ/mol·K; Δ G D o = 29.1 kJ/mol). Biotechnol. Bioeng. 2007;96:702–711. © 2006 Wiley Periodicals, Inc.