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Detecting oxygen consumption in the proximity of Saccharomyces cerevisiae cells using self‐assembled fluorescent nanosensors
Author(s) -
Kuang Yina,
Walt David R.
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21092
Subject(s) - nanosensor , fluorescence , chemistry , polyethylenimine , oxygen , nanotechnology , saccharomyces cerevisiae , biophysics , biochemistry , yeast , materials science , biology , organic chemistry , transfection , physics , quantum mechanics , gene
Abstract We describe a strategy for the preparation and self‐assembly of fluorescent nanosensors onto Saccharomyces cerevisiae cell surfaces for dynamically measuring oxygen concentration in the proximity of living cells. Amine functionalized polystyrene nanobeads were impregnated with an oxygen‐sensitive ruthenium(II) complex and the beads' surface was coated with polyethylenimine. The resulting nanosensors were assembled on individual S. cerevisiae cells in a controlled manner at physiological pH for continuously monitoring oxygen consumption. This approach exemplifies a general scheme for assembling fluorescent nanosensors on cells for the non‐invasive, reversible, and real‐time measurement of other physiologically relevant processes, such as the efflux of protons and carbon dioxide, or the influx of glucose. Biotechnol. Bioeng. 2007;96: 318–325. © 2006 Wiley Periodicals, Inc.