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Comparison of several Nicotiana species as hosts for high‐scale Agrobacterium ‐mediated transient expression
Author(s) -
Sheludko Y.V.,
Sindarovska Y.R.,
Gerasymenko I.M.,
Bannikova M.A.,
Kuchuk N.V.
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21075
Subject(s) - nicotiana benthamiana , green fluorescent protein , agrobacterium , biology , recombinant dna , nicotiana , transient (computer programming) , microbiology and biotechnology , botany , transgene , solanaceae , gene , genetics , computer science , operating system
Agrobacterium ‐mediated transient expression may be regarded as a promising method for inexpensive large‐scale production of recombinant proteins. We optimized the protocol of transient expression in Nicotiana benthamiana and compared six Australian species of Nicotiana as hosts for transient expression. The transient expression of GFP under 35S CaMV promoter was observed in all species tested, although the GFP content in leaves of N. benthamiana, N. exigua , and N. excelsior was significantly higher (3.8, 3.7, and 2.0% TSP, respectively). Usage of viral‐based expression system resulted in considerable increase of GFP accumulation in N. excelsior and N. benthamiana (63.5 and 16.2% TSP, respectively). We displayed that N. excelsior has the best characteristics in regard to biomass yield as well as GFP accumulation level for both types of the expression cassettes tested. Biotechnol. Bioeng. 2007;96: 608–614. © 2006 Wiley Periodicals, Inc.