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Biodegradable cationic polyester as an efficient carrier for gene delivery to neonatal cardiomyocytes
Author(s) -
Xu Peisheng,
Li ShiYan,
Li Qun,
Ren Jun,
Van Kirk Edward A.,
Murdoch William J.,
Radosz Maciej,
Shen Youqing
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.21036
Subject(s) - cationic polymerization , gene delivery , transfection , chemistry , genetic enhancement , in vitro , dissociation (chemistry) , polymer , green fluorescent protein , polyester , biophysics , gene , biochemistry , microbiology and biotechnology , polymer chemistry , biology , organic chemistry
Viral‐mediated gene delivery has been explored for the treatment and protection of cardiomyocytes, but so far there is only one report using cationic polymer for gene delivery to cardiomyocytes in spite of many advantages of polymer‐mediated gene delivery. In this study, a cationic poly(β‐amino ester) (PDMA) with a degradable backbone and cleavable side chains was synthesized by Michael addition reaction. The toxicity of PDMA to neonatal mouse cardiomyocytes (NMCMs) was significantly lower than that of polyethyleneimine (PEI). PDMA formed stable polyplexes with pEGFP. The dissociation of the polyplexes could be triggered by PDMA degradation, and the dissociation time was tunable via the polymer/pEGFP ratio. In vitro transfection showed that PDMA was an effective and low toxic gene delivery carrier for NMCMs. The PDMA/pEGFP polyplexes transfected EGFP gene to NMCMs with about 28% efficiency and caused little death. In contrast, a significant portion of cardiomyocytes cultured with PEI/pEGFP died. © 2006 Wiley Periodicals, Inc.