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Closure effects on oxygen transfer and aerobic growth in shake flasks
Author(s) -
Nikakhtari Hossein,
Hill Gordon A.
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20930
Subject(s) - laboratory flask , oxygen , pseudomonas putida , mass transfer , bioreactor , chemistry , yield (engineering) , chromatography , thermodynamics , biochemistry , organic chemistry , physics , enzyme
Oxygen mass transfer in shake flasks is an important aspect limiting the culture of aerobic microorganisms. In this work, mass transfer of oxygen through a closure and headspace of shake flasks is investigated. New equations for prediction of k G a in shake flasks with closures are introduced. Using Pseudomonas putida , microbial growth on glucose (fast metabolism) and phenol (slow metabolism) in shake flasks with closures were studied, considering both substrate and oxygen restrictions. A combined model for oxygen mass transfer and microbial growth is shown to accurately predict experimental oxygen concentrations and oxygen yield factors during growth experiments more accurately than previous models. © 2006 Wiley Periodicals, Inc.