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Enhanced lycopene production in Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate
Author(s) -
Yoon SangHwal,
Lee YoungMi,
Kim JuEun,
Lee SookHee,
Lee JooHee,
Kim JaeYean,
Jung KyungHwa,
Shin YongChul,
Keasling Jay D.,
Kim SeonWon
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20912
Subject(s) - lycopene , mevalonate pathway , escherichia coli , biochemistry , carotenoid , mevalonic acid , reductase , bacteria , biosynthesis , metabolic engineering , glycerol , chemistry , recombinant dna , enzyme , food science , biology , gene , genetics
Abstract To increase expression of lycopene synthetic genes crtE , crtB , crtI , and ipiHP1 , the four exogenous genes were cloned into a high copy pTrc99A vector with a strong trc promoter. Recombinant Escherichia coli harboring pT‐LYCm4 produced 17 mg/L of lycopene. The mevalonate lower pathway, composed of mvaK1 , mvaK2 , mvaD , and idi , was engineered to produce pSSN12Didi for an efficient supply of the lycopene building blocks, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Mevalonate was supplied as a substrate for the mevalonate lower pathway. Lycopene production in E . coli harboring pT‐LYCm4 and pSSN12Didi with supplementation of 3.3 mM mevalonate was more than threefold greater than bacteria with pT‐LYCm4 only. Lycopene production was dependent on mevalonate concentration supplied in the culture. Clump formation was observed as cells accumulated more lycopene. Further clumping was prevented by adding the surfactant Tween 80 0.5% (w/v), which also increased lycopene production and cell growth. When recombinant E . coli harboring pT‐LYCm4 and pSSN12Didi was cultivated in 2YT medium containing 2% (w/v) glycerol as a carbon source, 6.6 mM mevalonate for the mevalonate lower pathway, and 0.5% (w/v) Tween 80 to prevent clump formation, lycopene production was 102 mg/L and 22 mg/g dry cell weight, and cell growth had an OD 600 value of 15 for 72 h. © 2006 Wiley Periodicals, Inc.