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Cartilaginous tissue formation from bone marrow cells using rotating wall vessel (RWV) bioreactor
Author(s) -
Ohyabu Yoshimi,
Kida Naoko,
Kojima Hiroko,
Taguchi Tetsushi,
Tanaka Junzo,
Uemura Toshimasa
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20892
Subject(s) - cartilage , aggrecan , bone marrow , glycosaminoglycan , bioreactor , chondrogenesis , anatomy , chemistry , staining , regeneration (biology) , proteoglycan , safranin , biomedical engineering , pathology , microbiology and biotechnology , biology , osteoarthritis , medicine , articular cartilage , alternative medicine , organic chemistry
This is the first successful report of the rapid regeneration of three‐dimensional large and homogeneous cartilaginous tissue from rabbit bone marrow cells without a scaffold using a rotating wall vessel (RWV) bioreactor, which simulates a microgravity environment for cells. Bone marrow cells cultured for 3 weeks in DMEM were resuspended and cultured for 4 weeks in the chondrogenic medium within the vessel. Large cylindrical cartilaginous tissue with dimensions of (1.25 ± 0.06) × (0.60 ± 0.08) cm (height × diameter) formed. Their cartilage marker expression was confirmed by mRNA expressions of aggrecan, collagen type I and II, and glycosaminoglycan (GAG)/DNA ratio. Their cartilaginous properties were demonstrated by toluidine blue, safranin‐O staining, and polarization. © 2006 Wiley Periodicals, Inc.