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Effect of inhibition treatment, type of inocula, and incubation temperature on batch H 2 production from organic solid waste
Author(s) -
ValdezVazquez Idania,
RíosLeal Elvira,
MuñozPáez Karla M.,
CarmonaMartínez Alessandro,
PoggiVaraldo Héctor M.
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20891
Subject(s) - mesophile , incubation , chemistry , organic acid , solvent , fermentation , thermophile , fermentative hydrogen production , organic solvent , incubation period , nuclear chemistry , food science , biochemistry , enzyme , bacteria , hydrogen production , biology , genetics , chemical engineering , engineering , biohydrogen , catalysis
Two types of induction treatments (heat‐shock pretreatment, HSP, and acetylene, Ac), inocula (meso and thermophilic) and incubation temperatures (37 and 55°C) were tested according to a full factorial design 2 3 with the aim of assessing their effects on cumulative H 2 production ( P H , mmol H 2 /mini‐reactor), initial H 2 production rate ( R i,H , µmol H 2 /(g VS i × h)), lag time ( T lag , h), and metabolites distribution when fermenting organic solid waste with an undefined anerobic consortia in batch mini‐reactors. Type of inocula did not have a significant effect on P H , T lag , and R i,H except for organic acids production: mini‐reactors seeded with thermophilic inocula had the highest organic acid production. Concerning the induction treatment, it was found that on the average Ac only affected in a positive way the P H and T lag . Thus, P H in Ac‐inhibited units (6.97) was 20% larger than those in HSP‐inhibited units (5.77). Also, Ac favored a shorter T lag for P H in comparison with HSP (180 vs. 366). Additionally, a positive correlation was found between H 2 and organic acid production. In contrast, solvent concentration in heat‐shocked mini‐reactors were slightly higher than in reactors spiked with Ac. Regarding the incubation temperature, on the average mesophilic temperature affected in a positive and very significant way P H (10.07 vs. 2.67) and R i,H (2.43 vs. 0.76) with minimum T lag (87 vs. 459). The positive correlation between H 2 and organic acids production was found again. Yet, incubation temperature did not seem to affect solvent production. A strong interaction was observed between induction treatment and incubation temperature. Thus, Ac‐inhibited units showed higher values of P H and R i,H than that HSP‐inhibited units only under thermophilic incubation. Contrary to this, HSP‐inhibited units showed the highest values of P H and R i,H only under mesophilic conditions. Therefore, the superiority of an induction treatment seems to strongly depend on the incubation temperature. © 2006 Wiley Periodicals, Inc.