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Increased recombinant protein production in Escherichia coli strains with overexpressed water‐forming NADH oxidase and a deleted ArcA regulatory protein
Author(s) -
Vemuri G.N.,
Eiteman M.A.,
Altman E.
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20853
Subject(s) - escherichia coli , overproduction , heterologous , recombinant dna , biochemistry , heterologous expression , biology , oxidase test , chemistry , microbiology and biotechnology , enzyme , gene
Glycolytic flux is increased and acetate production is reduced in Escherichia coli by the expression of heterologous NADH oxidase (NOX) from Streptococcus pneumoniae coupled with the deletion of the arcA gene, which encodes the ArcA regulatory protein. In this study, we examined the overproduction of a model recombinant protein in strains of E. coli expressing NOX with or without an arcA mutation. The presence of NOX or the absence of ArcA reduced acetate by about 50% and increased β‐galactosidase production by 10–20%. The presence of NOX in the arcA strain eliminated acetate production entirely in batch fermentations and resulted in a 120% increase in β‐galactosidase production. © 2006 Wiley Periodicals, Inc.