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On the influence of oxygen and cell concentration in an SFPR whole cell biocatalytic Baeyer–Villiger oxidation process
Author(s) -
Hilker Iris,
Baldwin Chris,
Alphand Véronique,
Furstoss Roland,
Woodley John,
Wohlgemuth Roland
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20829
Subject(s) - bioprocess , schematic , process (computing) , biochemical engineering , oxidation process , chemistry , baeyer–villiger oxidation , oxygen , substrate (aquarium) , process engineering , computer science , organic chemistry , chemical engineering , biology , catalysis , engineering , ecology , electronic engineering , operating system
Efficient whole cell biotransformations, in particular microbial whole cell Baeyer–Villiger oxidation with molecular oxygen, demand comprehension and optimization of the process details involved. Optimal provision of oxygen and control of bioprocess parameters are pivotal for their success. The interrelation of cell density and oxygen supply in an in situ substrate feeding and product removal (SFPR) whole cell Baeyer–Villiger oxidation process was investigated in detail. Both parameters were optimized with respect to practical considerations. The outcome of this study supports a schematic process model, allows estimation of optimum process conditions and exploration of its limits. © 2006 Wiley Periodicals, Inc.