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Influence of perfusion on metabolism and matrix production by bovine articular chondrocytes in hydrogel scaffolds
Author(s) -
Xu Xia,
Urban Jill P.G.,
Tirlapur Uday,
Wu MinHsein,
Cui Zheng,
Cui Zhanfeng
Publication year - 2006
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20818
Subject(s) - glycosaminoglycan , agarose , perfusion , extracellular matrix , cartilage , chemistry , scaffold , biomedical engineering , microbiology and biotechnology , matrix (chemical analysis) , tissue engineering , tissue culture , biophysics , in vitro , anatomy , biochemistry , biology , chromatography , medicine
Articular cartilage has a limited capacity for self‐repair after damage. Engineered cartilage is a promising treatment to replace or repair damaged tissue. The growth of engineered cartilage is sensitive to the extracellular culture environment. Chondrocytes were seeded into alginate beads and agarose scaffolds at 4 millions/mL, and the response to static and perfusion culture was examined over period of up to 12 days. For both types of scaffolds, the chondrocytes kept their differentiated morphology over 12 days in all culture conditions. In alginate beads, more glycosaminoglycans (GAGs) were produced in perfusion culture than in static conditions. GAG distribution in alginate constructs was more uniform in perfusion culture than in static culture. However, in agarose constructs there was no significant difference in GAG production between static culture and perfusion culture. Under perfusion culture, the retention rate of GAG in alginate was higher than in agarsoe. It is suggested that the positive effect of perfusion culture only can be achieved by an appropriate choice of other factors such as scaffold materials. © 2006 Wiley Periodicals, Inc.