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The cold‐shock response in cultured mammalian cells: Harnessing the response for the improvement of recombinant protein production
Author(s) -
AlFageeh Mohamed B,
Marchant Rosalyn J,
Carden Martin J,
Smales C Mark
Publication year - 2005
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20789
Subject(s) - recombinant dna , microbiology and biotechnology , protein biosynthesis , cold shock domain , biology , cell culture , cytoskeleton , translation (biology) , cell , biochemistry , messenger rna , gene , rna , genetics
There are a growing number of reports on the sub‐physiological temperature culturing (<37°C) of mammalian cells for increased recombinant protein yield, although the effect is variable between cell lines, expression systems, and the product of interest. What is becoming clear is that exposing mammalian cells to sub‐physiological temperatures invokes a coordinated cellular response involving modulation of the cell cycle, metabolism, transcription, translation, and the cell cytoskeleton. Opportunities currently exist for further enhancement of the cold‐shock effect on recombinant protein production in mammalian cells through advancements in our understanding of the mechanisms involved in the cold‐shock response. © 2005 Wiley Periodicals, Inc.