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Optimization of the cell seeding density and modeling of cell growth and metabolism using the modified Gompertz model for microencapsulated animal cell culture
Author(s) -
Wentao Qi,
Ying Zhang,
Juan Ma,
Xin Guo,
Yubing Xie,
Wei Wang,
Xiaojun Ma
Publication year - 2005
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20782
Subject(s) - gompertz function , cell growth , seeding , cell culture , chemistry , substrate (aquarium) , cell , cell metabolism , in vivo , biochemical engineering , microbiology and biotechnology , biological system , biophysics , biochemistry , biology , computer science , agronomy , engineering , ecology , genetics , machine learning
Cell microencapsulation is one of the promising strategies for the in vitro production of proteins or in vivo delivery of therapeutic products. In order to design and fabricate the optimized microencapsulated cell system, the Gompertz model was applied and modified to describe the growth and metabolism of microencapsulated cell, including substrate consumption and product formation. The Gompertz model successfully described the cell growth kinetics and the modified Gompertz models fitted the substrate consumption and product formation well. It was demonstrated that the optimal initial cell seeding density was about 4–5 × 10 6 cells/mL of microcapsule, in terms of the maximum specific growth rate, the glucose consumption potential and the product formation potential calculated by the Gompertz and modified Gompertz models. Modeling of cell growth and metabolism in microcapsules provides a guideline for optimizing the culture of microencapsulated cells. © 2005 Wiley Periodicals, Inc.