Exploitation of the alcohol dehydrogenase‐acetone NADP‐regeneration system for the enzymatic preparative‐scale production of 12‐ketochenodeoxycholic acid | Zendy

Fossati Elena | Zendy; Polentini Fausto | Zendy; Carrea Giacomo | Zendy; Riva Sergio | Zendy

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Exploitation of the alcohol dehydrogenase‐acetone NADP‐regeneration system for the enzymatic preparative‐scale production of 12‐ketochenodeoxycholic acid

Author(s) -

Fossati Elena,

Polentini Fausto,

Carrea Giacomo,

Riva Sergio

Publication year - 2005

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.20753

Subject(s) - acetone , alcohol dehydrogenase , chemistry , dehydrogenase , substrate (aquarium) , cholic acid , regioselectivity , enzyme , isocitrate dehydrogenase , alcohol , organic chemistry , chromatography , biochemistry , catalysis , biology , bile acid , ecology

The performance of a new NADP‐regeneration system, based on the use of alcohol dehydrogenase (ADH)‐acetone, has been investigated for the regioselective oxidation of cholic acid ( 1 ) to 12‐ketochenodeoxycholic acid ( 2 ). Enzymes stabilities and substrate and/or product inhibitory effects under defined synthetic reaction conditions have been evaluated. The optimized system, based on a 4% w/v solution of 1 in a reaction mixture containing 25% v/v acetone, allowed the preparative scale transformation of 1 into 2 with a 92% conversion. © 2005 Wiley Periodicals, inc.

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