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Exploitation of the alcohol dehydrogenase‐acetone NADP‐regeneration system for the enzymatic preparative‐scale production of 12‐ketochenodeoxycholic acid
Author(s) -
Fossati Elena,
Polentini Fausto,
Carrea Giacomo,
Riva Sergio
Publication year - 2005
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20753
Subject(s) - acetone , alcohol dehydrogenase , chemistry , dehydrogenase , substrate (aquarium) , cholic acid , regioselectivity , enzyme , isocitrate dehydrogenase , alcohol , organic chemistry , chromatography , biochemistry , catalysis , biology , bile acid , ecology
The performance of a new NADP‐regeneration system, based on the use of alcohol dehydrogenase (ADH)‐acetone, has been investigated for the regioselective oxidation of cholic acid ( 1 ) to 12‐ketochenodeoxycholic acid ( 2 ). Enzymes stabilities and substrate and/or product inhibitory effects under defined synthetic reaction conditions have been evaluated. The optimized system, based on a 4% w/v solution of 1 in a reaction mixture containing 25% v/v acetone, allowed the preparative scale transformation of 1 into 2 with a 92% conversion. © 2005 Wiley Periodicals, inc.