Prevention of thermal induced aggregation of cytochrome  c  at isoelectric pH values by polyanions | Zendy

Fedunová Diana | Zendy; Antalík Marián | Zendy

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Prevention of thermal induced aggregation of cytochrome c at isoelectric pH values by polyanions

Author(s) -

Fedunová Diana,

Antalík Marián

Publication year - 2006

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.20733

Subject(s) - differential scanning calorimetry , isoelectric point , chemistry , viscometer , sulfonate , cytochrome c , isoelectric focusing , protein aggregation , chromatography , organic chemistry , biochemistry , viscosity , materials science , sodium , thermodynamics , physics , mitochondrion , composite material , enzyme

Differential scanning calorimetry, viscometry, optical and CD spectroscopy were used to characterize the influence of two polyanions, poly(vinylsulfate) (PVS), and poly(4‐styrene‐sulfonate) (PSS) on thermal transition reversibility of ferricytochrome c at or near isoelectric pH. In these conditions, both PVS and PSS enhance the thermal transition reversibility of cytochrome c by preventing the aggregation of denatured protein molecules. Data indicate that the polyanions are in complex with cytochrome c that is stabilized by synergistic effect of Coulombic and non‐Coulombic interactions. © 2005 Wiley Periodicals, Inc.

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