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Beneficial effect of silkworm hemolymph on a CHO cell system: Inhibition of apoptosis and increase of EPO production
Author(s) -
Choi Shin Sik,
Rhee Won Jong,
Park Tai Hyun
Publication year - 2005
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.20550
Subject(s) - sodium butyrate , chinese hamster ovary cell , apoptosis , hemolymph , erythropoietin , butyrate , biochemistry , biology , cell culture , microbiology and biotechnology , chemistry , endocrinology , fermentation , receptor , genetics , gene
To produce erythropoietin (EPO), Chinese hamster ovary (CHO) cells were first cultured in a medium containing FBS (growth medium) and then in a serum‐free medium containing sodium butyrate (production medium). Sodium butyrate increases recombinant protein production, but also induces apoptosis, which reduces cell viability and productivity. In a previous study, we found that silkworm hemolymph (SH), an insect serum, inhibits the apoptosis of insect and mammalian cells. To overcome sodium butyrate‐induced apoptosis, we added SH to growth medium. This pretreatment with SH inhibited the sodium butyrate‐induced apoptosis of CHO cells and consequently increased their longevity and their ability to produce EPO. As a result, the volumetric productivity of EPO was increased five‐fold. SH was found to inhibit cytochrome c release from mitochondria into the cytosol, and prevented the activation of caspase‐3 and other subsequent caspase reactions. © 2005 Wiley Periodicals, Inc.